Composite

Part:BBa_K1878002

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


pBAD/His/iRFP670

Usage and Biology

Arabinose inducible iRFP 670 with His-tag

The original citation is as follows: The original citation is as follows: D.M. Shcherbakova and V.V. Verkhusha. "Near-infrared fluorescent proteins for multicolor in vivo imaging." Nature Methods, 2013. 10(8): p. 751-4.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2271
    Illegal NheI site found at 1260
    Illegal NheI site found at 1415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2271
    Illegal BglII site found at 1329
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1316
    Illegal XhoI site found at 1325
    Illegal XhoI site found at 1884
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2271
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2271
    Illegal NgoMIV site found at 1464
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1970
    Illegal BsaI site found at 2450
    Illegal BsaI.rc site found at 1527
    Illegal SapI site found at 961


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Categories
Parameters
None